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Anti-GAD2 Antibody EPR2553Y
Also for GAD2 (NM_000818)
|A synthetic peptide corresponding to residues in human GAD2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500 - 1:2000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2), transcript variant 1|
|This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]. |
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Western blot - GAD65 antibody [EPR2553Y]; All lanes : Anti-GAD65 antibody [EPR2553Y] at 1/1000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : Mouse brain lysate.Lane 3 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 65 kDa.Observed band size : 70 kDa .Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.