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Anti-FTL Antibody EPR5260

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307874
  • Rabbit monoclonal antibody against Ferritin (light chain)(clone EPR5260)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400051) , 20ug
100ul $325 In Stock
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WB(1)
FC(1)
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Also for FTL (NM_000146)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus in human Ferritin was used as an immunogen. This antibody recognizes the light chain only.
Clone NameEPR5260 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens ferritin, light polypeptide (FTL)
Alternative NameLFTD; NBIA3
Database LinkNP_000137
FunctionFerritin is important in iron homeostasis. It is the main iron-storage protein, composed of two partially homologous subunits, heavy and light chains. Ferritin molecules in cells containing high levels of iron tend to be rich in light chains and may have a long-term storage function, whereas heavy-rich ferritins are more active in iron metabolism (1, 2). Mutations in ferritin cause hereditary hyperferritinemia-cataract syndrome and neuroferritinopathy, associated with inflammation. Elevated levels of ferritin are reported as characteristics of adult-onset Still's disease and hemophagocytic syndrome, also associated with inflammation (3).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Ferritin Light Chain antibody [EPR5260]; All lanes : Anti-Ferritin Light Chain antibody [EPR5260] at 1/10000 dilution.Lane 1 : Fetal liver lysate.Lane 2 : HepG2 cell lysate.Lane 3 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 20 kDa.Observed band size : 19 kDa .
FC Image
Flow Cytometry - Ferritin Light Chain antibody [EPR5260]; Flow cytometric analysis of permeabilized HeLa cells using TA307874 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

 

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