Home Antibody All anti-FRAP1 antibodies
Also for FRAP1 (NM_004958)
|This mTOR (FRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2459-2488 amino acids from human mTOR (FRAP1).|
|Human (Predicted: Mouse, Rat)
|WB, IHC, IF
||WB: 1:1000, IHC: 1:10~50, IF: 1:10~50
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is purified through a protein A column, followed by peptide affinity purification.
|Homo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR)|
|FRAP; FRAP1; FRAP2; RAFT1; RAPT1|
Entrez Gene 2475 Human
Entrez Gene 56717 Mouse
Entrez Gene 56718 Rat
|FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.|
Senescence and Autophagy
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Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481) (Cat.#TA302216). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human testis tissue reacted with FRAP1-pS2481, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of mTOR (FRAP1) Antibody (S2481)(Cat#TA302216) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).