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Anti-FOXO3 Antibody EP1949Y
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Also for FOXO3 (NM_001455)
|A synthetic peptide corresponding to residues near the N-terminus of human FOXO3a was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens forkhead box O3 (FOXO3), transcript variant 1|
|AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A|
Entrez Gene 2309 Human
|Forkhead box O-class (FOXO) transcription factors are mammalian homologue of DAF-16, a known lifespan regulator of Caenorhabditis elegans. FOXO transcription factors, such as FOXO1, FOXO3a, and FOXO4, have been linked to the regulation of cell cycle, apoptosis, DNA repair, oxidative stress, and metabolism (1). FOXO proteins are proposed to be dual regulated by Akt-mediated phosphorylation and Skp2-mediated ubiquitination, which are usually initiated by growth factors and cytokines. FOXO proteins are also activated by stress, which induces gene expression that contributes to cell cycle arrest, implicating FOXO proteins as tumor suppressors (2). Specifically, FOXO3a (FKHR-L1) is a transcriptional activator for apoptosis in the absence of survival factors, such as in neural cell death triggered by oxidative stress. In the presence of survival factors like IGF-1, AKT1/PKB phosphorylates on Thr-32 and Ser-253. However, in the absence of survival factors, FOXO3a is dephospholyated and translocated to the nucleus inducing transcription of target genes and apoptosis (3). |
|Transcription FactorsDruggable Genome Chemokine signaling pathwayNeurotrophin signaling pathwayEndometrial cancerNon-small cell lung cancer|
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Western blot - FOXO3A antibody [EP1949Y]; Anti-FOXO3A antibody [EP1949Y] at 1/5000 dilution + Jurkat cell lysate at 10 ug.Secondary.Goat anti-Rabbit HRP labeled at 1/2000 dilution.Predicted band size : 71 kDa.Observed band size : 90 kDa .
Immunohistochemistry (Paraffin-embedded sections) - FOXO3A antibody [EP1949Y]; Ab53287 (1:50) staining human FOXO3A in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.