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Anti-FLT3LG Antibody EP1140Y
Also for FLT3LG (NM_001459)
|A synthetic peptide corresponding to residues within human Flt3-L was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:100,000; IHC: 1:50 100; ICC: 1:100 250; FC: 1:10
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens fms-related tyrosine kinase 3 ligand (FLT3LG), transcript variant 3|
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
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Western blot - Flt3 ligand antibody [EP1140Y]; Anti-Flt3 ligand antibody [EP1140Y] at 1/100000 dilution + Recombinant Flr3 ligand at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 26 kDa.Observed band size : 22 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Flt3 ligand antibody [EP1140Y]; ab52648 stainingFlt3 ligandin Rhesus monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (1/100 in 20% Normanl Goat serum in 1x TTBS) for 12 hours at 20°C. A Biotin-conjugatedGoat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Immunohistochemistry (Paraffin-embedded sections) - Flt3 ligand antibody [EP1140Y]; Immunohistochemical staining of paraffin-embedded human tonsils using ab52648 at a 1/50 dilution.