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Anti-FLII Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA308275 Rabbit Polyclonal antibody to Flightless I (flightless I homolog (Drosophila)) 100ul $325 3-7 Days
LC419581 FLII HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 435 and 778 of Flightless I (Uniprot ID#Q13045)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Chicken, Rat, Xenopus tropicalis) Concentration0.88mg/ml
Guaranteed Application *WB, IF Suggested DilutionsICC/IF:1:100-1:1000; WB:1:500-1:3000
Predicted MW Explanation 145kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Chicken (87%), Rat (96%), Xenopus tropicalis (81%)

Reference Data

Target NameHomo sapiens flightless I homolog (Drosophila) (FLII), transcript variant 1
Alternative NameFLI; Fli1; FLIL
Database LinkNP_002009
Entrez Gene 2314 Human
Entrez Gene 14248 Mouse
Entrez Gene 287375 Rat
FunctionThis gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]
Related PathwayES Cell Differentiation/IPSTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. TA308275 diluted at 1:1000.

WB Image
Sample(30 ug whole cell lysate). A:Raji . 5% SDS PAGE. TA308275 diluted at 1:1000

IF Image
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Flightless I(TA308275) antibody at 1:200 dilution.

IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless I(TA308275) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.


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