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Home Antibody All anti-FH antibodies
UltraMAB

Anti-FH GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590010 Rabbit Polyclonal Fumarase Antibody 100ug $325 In Stock
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WB(2)
IHC(5)
IF(1)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration0.79mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens fumarate hydratase (FH)
Alternative NameFMRD; HLRCC; LRCC; MCL; MCUL1
Database LinkNP_000134
Entrez Gene 2271 Human
FunctionThe protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
Related PathwayDruggable Genome Citrate cycle (TCA cycle)Metabolic pathwaysPathways in cancerRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (RC200614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
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Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
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ELISA: Fumarase Antibody
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
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Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FH Rabbit Polyclonal antibody. (TA590010)
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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
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Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
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