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Home Antibody All anti-FERMT2 antibodies

Anti-FERMT2 TRUEMAB Antibody clone 14A11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500505
  • anti-FERMT2 mouse monoclonal antibody, clone 14A11
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416377) , 20ug
100ul 325 In Stock
CF500505
  • Carrier-free (BSA/glycerol-free) FERMT2 mouse monoclonal antibody, clone 14A11
100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(1)
IF(2)
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Also for FERMT2 (NM_006832)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human FERMT2 expression vector
Clone Nameclone 14A11 IsotypeIgG2a
Species ReactivityHuman, Dog, Rat, Monkey Concentration1
Guaranteed Application *WB, IHC, IF, IP Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:50~100, IP 2ug/500ul
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens fermitin family member 2 (FERMT2), transcript variant 1
Alternative NameKIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B
Database LinkNP_006823
FunctionParticipates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FERMT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500505)
IF Image
Anti-FERMT2 mouse monoclonal antibody (TA500505) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).
IF Image
Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500505).
IP Image
Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

 

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