Anti-FERMT2 Antibody, clone 14A11

Also for FERMT2 (NM_006832)

Gene Name	Homo sapiens fermitin family member 2 (FERMT2), transcript variant 1
Synonyms:	KIND2; mig-2; MIG2; PLEKHC1; UNC112; UNC112B
Immunogen	Protein expressed in 293T cell transfected with human FERMT2 expression vector
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 14A11	Isotype	IgG2a
Species Reactivity	Human, Dog, Rat, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, IP	Suggested Dilutions	WB 1:1000~2000, IHC 1:50, IF 1:50~100, IP 2ug/500ul
Background	Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FERMT2 monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-FERMT2 mouse monoclonal antibody. (TA500505)

IF Image

Anti-FERMT2 mouse monoclonal antibody (TA500505) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2(RC205727).

IF Image

Immunofluorescent staining of HepG2 cells using anti-FERMT2 mouse monoclonal antibody (TA500505).

IP Image

Immunoprecipitation(IP) of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Anti-FERMT2 TrueMAB Antibody clone 14A11

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