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Home Antibody All anti-FEN1 antibodies

Anti-FEN1 Antibody EPR4459(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307793
  • Rabbit monoclonal antibody against FEN1(clone EPR4459(2))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401328) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(2)
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Also for FEN1 (NM_004111)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human FEN1 was used as an immunogen.
Clone NameEPR4459(2) IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IP.

Reference Data

Target NameHomo sapiens flap structure-specific endonuclease 1 (FEN1)
Alternative NameFEN-1; MF1; RAD2
Database LinkNP_004102
FunctionFEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. FEN1 is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by FEN1. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions (1).
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-FEN1 antibody [EPR4459(2)]; All lanes : Anti-FEN1 antibody [EPR4459(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)]; Immunohistochemical analysis of paraffin-embedded Human breast tissue labelled with TA307793 at 1/500 dilution.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)]; Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelled with TA307793 at 1/500 dilution.

 

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