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Home Antibody All anti-FEN1 antibodies

Anti-FEN1 Antibody EPR4459(2)

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SKU Description Amount Price Availability*  
TA307793
  • Rabbit monoclonal antibody against FEN1(clone EPR4459(2))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401328) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(2)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human FEN1 was used as an immunogen.
Clone NameEPR4459(2) IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000
Predicted MW Explanation 43 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,ICC or IP.

Reference Data

Target NameHomo sapiens flap structure-specific endonuclease 1 (FEN1)
Alternative NameFEN-1; MF1; RAD2
Database LinkNP_004102
Entrez Gene 2237 Human
Entrez Gene 14156 Mouse
Entrez Gene 84490 Rat
FunctionFEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. FEN1 is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by FEN1. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions (1).
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNon-homologous end-joining

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-FEN1 antibody [EPR4459(2)]; All lanes : Anti-FEN1 antibody [EPR4459(2)] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 43 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)]; Immunohistochemical analysis of paraffin-embedded Human breast tissue labelled with TA307793 at 1/500 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4459(2)]; Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labelled with TA307793 at 1/500 dilution.
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