Home Antibody All anti-FANCC antibodies
Also for FANCC (NM_000136)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 96-112 of Human FANCC.|
||ELISA: 1:20,000 - 1:80,000, WB: 1:1,000 - 1:3,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|FANCC (also called Protein FACC or Fanconi Anemia Group C protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCC may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. FANCC belongs to the multi-subunit Fanconi Anemia (FA) complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. FANCC is mainly found within the nucleus although some protein is localized in the cytoplasm. This protein is ubiquitously expressed. Defects in FANCC are a cause of Fanconi anemia (FA). FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopaenia, a diverse assortment of congenital malformations, and
|Homo sapiens Fanconi anemia, complementation group C (FANCC), transcript variant 1|
|FA3; FAC; FACC|
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Western blot using affinity purified anti-FANCC antibody shows detection of a band at ~63 kDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate. The identity of the lower molecular weight band is unknown. Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,500 in PBS supplemented with 1% normal goat serum and 0.1% BLOTTO (B501-0500). The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.