Home Antibody All anti-FANCA antibodies
Also for FANCA (NM_000135)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 995-1009 of human FANCA protein.|
||ELISA: 1:15,000 - 1:60,000, WB: 1:500 - 1:3,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|FANCA (also called Protein FACA or Fanconi anemia group A protein) is involved in DNA repair, perhaps specifically with post-replication repair or a cell cycle checkpoint function. FANCA may also be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, and FANCL. The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia
|Homo sapiens Fanconi anemia, complementation group A (FANCA), transcript variant 1|
|FA; FA-H; FA1; FAA; FACA; FAH; FANCH|
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Western blot using affinity purified anti-FANCA antibody shows detection of FANCA only in FANCA transfected GM6914 cell lysates. No staining is seen in lysates prepared from FANCA (-/-) cells in the absence of FANCA transfection. Modified from Smogorzewska et al (2007) Cell 129, 289-301.
Western blot using affinity purified anti-FANCA antibody shows detection of a band at ~133 kDa (arrowhead) corres-ponding to FANCA in HeLa whole cell lysates. The identity of the lower molecular weight bands is unknown but may represent breakdown products. Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed for 2 h at room temperature with the primary antibody diluted to 1:1,500. The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red). IRDye™800 fluorescence images were captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.