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Anti-F9 Antibody EPR6187
|A synthetic peptide corresponding to residues in human Factor IX was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; FC: 1:10 - 1:100; ICC/IF: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Is unsuitable for IHC-P or IP.
|Homo sapiens coagulation factor IX (F9)|
|FIX; HEMB; P19; PTC; THPH8|
|Factor IX is vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this protein, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease (1). |
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Western blot - Anti-Factor IX antibody [EPR6187]; All lanes : Anti-Factor IX antibody [EPR6187] at 1/1000 dilution.Lane 1 : HepG2 cell lysate .Lane 2 : Human plasma .Lysates/proteins at 10 µg per lane.Predicted band size : 52 kDa.
Other-Anti-Factor IX antibody [EPR6187](TA310474); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-Factor IX antibody [EPR6187]; TA310474, at 1/10 dilution, staining Factor IX in permeabilized HepG2 cells (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.