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Anti-ESRRA Antibody EPR46Y
Also for ESRRA (NM_004451)
|A synthetic peptide corresponding to residues near the N-terminus of human ERRalpha was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IP: Use at an assay dependent concentration; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for ICC or IHC-P.
|Homo sapiens estrogen-related receptor alpha (ESRRA), transcript variant 1|
|ERR1; ERRa; ERRalpha; ESRL1; NR3B1|
|The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. [provided by RefSeq]. |
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Western blot - Estrogen Related Receptor alpha antibody [EPR46Y]; Anti-Estrogen Related Receptor alpha antibody [EPR46Y] at 1/5000 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 55 kDa.Observed band size : 55 kDa.
Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y]; Estrogen Related Receptor alpha was immunoprecipitated using 0.5mg Hela whole cell extract, 10ug of Rabbit monoclonal to Estrogen Related Receptor alpha and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). .The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70oC; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA303506.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) .Band: Band: 55kDa: Estrogen Related Receptor alpha.