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Anti-ERCC1 TRUEMAB Antibody clone 1C10
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for ERCC1 (NM_001983)
|Full length human recombinant protein of human ERCC1(NP_001974) produced in HEK293T cell.|
|Human , Mouse
|WB, IF, FC
|| WB 1:500~2000, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 2|
|COFS4; RAD10; UV20|
Entrez Gene 2067 Human
Entrez Gene 13870 Mouse
|The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq, Oct 2009]. |
|Druggable Genome Nucleotide excision repair|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERCC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-ERCC1 mouse monoclonal antibody (TA504365) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504365), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-ERCC1 antibody(TA504365),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody(TA504365),(Red), compared to a nonspecific negative control antibody,(Blue).