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Anti-ERCC1 TRUEMAB Antibody Clone OTI2A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA504287 ERCC1 mouse monoclonal antibody, clone OTI2A8 (formerly 2A8) 100ul 325
In Stock
LC419605 ERCC1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504287 Carrier-free (BSA/glycerol-free) ERCC1 mouse monoclonal antibody, clone OTI2A8 (formerly 2A8) 100ug $450 3-4 weeks
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Also for ERCC1 (NM_001983)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ERCC1(NP_001974) produced in HEK293T cell.
Clone NameClone OTI2A8 IsotypeIgG1
Species ReactivityHuman Concentration0.47 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100,
Predicted MW Explanation 32.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens excision repair cross-complementation group 1 (ERCC1), transcript variant 2
Alternative NameCOFS4; RAD10; UV20
Database LinkNP_001974
Entrez Gene 2067 Human
FunctionThe product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq].
Related PathwayDruggable Genome Nucleotide excision repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

IF Image
Anti-ERCC1 mouse monoclonal antibody (TA504287) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).

FC Image
HEK293T cells transfected with either RC200478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERCC1 antibody(TA504287), and then analyzed by flow cytometry.


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