Anti-ERCC1 Antibody, clone 3F2

Also for ERCC1 (NM_001983)

OriGene Data

Immunogen	Full length human recombinant protein of human ERCC1 (NP_001974) produced in HEK293T cell.
Clone Name	clone 3F2	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.04% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 2
Alternative Name	COFS4; RAD10; UV20
Database Link	NP_001974
Function	The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1.

IHC Image

Immunohistochemical staining of paraffin-embedded liver tissue using anti-ERCC1 mouse monoclonal antibody. (TA501168, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-ERCC1 mouse monoclonal antibody. (TA501168, Dilution 1:50)

IF Image

Anti-ERCC1 mouse monoclonal antibody (TA501168) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1(RC200478).

FC Image

HEK293T cells transfected with either pCMV6-ENTRY ERCC1(RC200478)(Red) or empty vector control plasmid(Blue) were immunostained with anti-ERCC1 mouse monoclonal(TA501168), and then analyzed by flow cytometry.

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Anti-ERCC1 TrueMAB Antibody clone 3F2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data