Anti-ERBB3 Antibody, EPR2325

Also for ERBB3 (NM_001005915)

OriGene Data

Immunogen	A phospho-specific peptide corresponding to residues surrounding Tyrosine 1289 of human HER3/ErbB3 was used as an immunogen. The antibody only detects HER3/ErbB3 phosphorylated on Tyrosine 1289.
Clone Name	EPR2325	Isotype	Rabbit IgG
Species Reactivity	Human	Concentration
Guaranteed Application *	WB, IF	Suggested Dilutions	WB: 1:2500 -5000; ICC: 1:100
Buffer	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) (ERBB3), transcript variant s
Alternative Name	c-erbB-3; c-erbB3; ErbB-3; erbB3-S; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3
Database Link	NP_001005915
Function	This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq].
Related Pathway

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WB Image

Western blot analysis on SKBR3 cell lysate using anti-Phospho-HER3/ErbB3 (pY1289) RabMAb (TA303500), 1:5000 dilution. Cells were either (A) untreated (B) treated with Neuregulin

IF Image

Immunofluorescent staining of MCF7 cells using phospho-specific anti-HER3/ErbB3 (pY1289) RabMAb (TA303500).

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OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-ERBB3 Phospho Antibody EPR2325

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data