UltraMAB™ Antibodies - Validated with 10K Protein Chip
Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (UM500034, clone UMAB34, 1:500).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ERBB2 monoclonal antibody (Clone UMAB34) at 1:500.
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-ERBB2mouse monoclonal antibody. (Clone UMAB34, Dilution 1:100)
Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++) using anti-ERBB2mouse monoclonal antibody. (Clone UMAB34, Dilution 1:100)
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody (Clone UMAB34). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 (Clone UMAB34) very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq].
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Western blot analysis of cell lysates of HEK293 transfected with ERBB2 cDNA (Left lane) or untransfected (Right lane) by using ERBB2 UltraMAB (UM500034, clone UMAB34, 1:500).
Immunohistochemical staining of paraffin-embedded Carcinoma of breast tissue (HER2+++) using anti-ERBB2mouse monoclonal antibody. (Clone UMAB34, Dilution 1:100)
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ERBB2 mouse monoclonal antibody (Clone UMAB34). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-ERBB2 (Clone UMAB34) very specifically recognizes ERBB2 antigen on OriGene protein microarray chip.
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