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Home Antibody All anti-ERBB2 antibodies

Anti-ERBB2 TRUEMAB Antibody clone 9G2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA503526
  • ERBB2 mouse monoclonal antibody,clone 9G2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417979) , 20ug Explanation
100ul 325 In Stock
CF503526
  • Carrier-free (BSA/glycerol-free) ERBB2 mouse monoclonal antibody,clone 9G2
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for ERBB2 (NM_004448)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 676-1255 of human ERBB2(NP_004439) produced in HEK293T cell.
Clone Nameclone 9G2 IsotypeIgG2b
Species ReactivityHuman Concentration0.71 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 137.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography
Note Antibody specificy evaluation by using OriGene Protein microarray chip and WB analysis showed that this antibody cross-reacts with CDH1 recombinant protein in overexpression lysates.

Reference Data

Target NameHomo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1
Alternative NameCD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Database LinkNP_004439
Entrez Gene 2064 Human
FunctionThis gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq].
Related PathwayProtein KinaseTransmembraneDruggable Genome ErbB signaling pathwayCalcium signaling pathwayFocal adhesionAdherens junctionPathways in cancerPancreatic cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERBB2 (RC212583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERBB2.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ERBB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503526)
IF Image
Anti-ERBB2 mouse monoclonal antibody (TA503526) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2(RC212583).
FC Image
HEK293T cells transfected with either RC212583 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ERBB2 antibody(TA503526), and then analyzed by flow cytometry.

 

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