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Anti-ERBB2 Antibody EP1045Y
Also for ERBB2 (NM_004448)
|A synthetic peptide corresponding to residues near the C-terminus of human Her2 was used as an immunogen. This antibody detects Her2 phosphorylated at Tyr1248 as well as unphosphorlyated Her2.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC, IF
||WB: 1:10000 - 1:50000; IP: 1:50; FC: 1:30; ICC/IF: 1:250 - 1:500; IHC-P: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (ERBB2), transcript variant 1|
|CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1|
|ErbB2/HER2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. |
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Western blot - Anti-ErbB 2 antibody [EP1045Y]; Anti-ErbB 2 antibody [EP1045Y] at 1/10000 dilution + SKBR-3 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 137 kDa.
Other-Anti-ErbB 2 antibody [EP1045Y](TA300959); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB 2 antibody [EP1045Y]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB 2 with TA300959 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ErbB 2 antibody [EP1045Y]; Immunofluorescent analysis of SKBR cells labelling ErbB 2 with TA300959 at 1/250 dilution.