Also for EPHB2 (NM_001127319)
|Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr298 conjugated to KLH|
||Lot dependent; please refer to CoA along with shipment
|100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
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Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr298 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: Î»-Ptase). The blot is identical to the control except that it was incubated in Î»-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr298 EphrinB. The immunolabeling of the EphrinB band is completely eliminated by treatment with Î»-Ptase.