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Anti-EphB1 Antibody EPR6457
Also for EphB1 (NM_004441)
|A synthetic peptide corresponding to residues in human EphB1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
|Homo sapiens EPH receptor B1 (EPHB1)|
|ELK; EPHT2; Hek6; NET|
|Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB1 is a receptor for ephrin-B family members.|
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Western blot - Anti-Eph receptor B1 antibody [EPR6457]; All lanes : Anti-Eph receptor B1 [EPR6457] antibody at 1/1000 dilution.Lane 1 : MDA-MB-435 cell lysate.Lane 2 : U-87MG cell lysate.Lane 3 : NIH 3T3 cell lysate.Lane 4 : COS1 cell lysate.Lane 5 : Human fetal brain cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 110 kDa.
Other-Anti-Eph receptor B1 antibody [EPR6457](ab129103); Equilibrium disassociation constant (KD)..
Immunocytochemistry/ Immunofluorescence - Anti-Eph receptor B1 antibody [EPR6457]; Immunofluorescence analysis of rat oligodendrocytes, staining Eph receptor B1 with ab129103. Cells were fixed with formaldehyde, permeabilized with 0.03% Triton X-100 and blocked with 1% serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300 in serum + 0.01% Triton X-100) for 10 hours at 4°C. An AlexaFluor?555-conjugated donkey anti-rabbit polyclonal IgG (1/300) was used as the secondary antibody.