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Anti-EPCAM GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for EPCAM (NM_002354)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein (NM_002354).|
|Human , Monkey
|WB, ELISA, IHC, IF
|| WB 1:5000~20000, IHC 1:150, IF 1:500,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens epithelial cell adhesion molecule (EPCAM)|
|DIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1|
Entrez Gene 4072 Human
Entrez Gene 677680 Monkey
|CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule inthe basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has beendetected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.|
|ES Cell Differentiation/IPSTransmembrane |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPCAM.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPCAM polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA: EpCAM Antibody 19300002 - Affinity Purified
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
Immunofluorescent staining of HeLa cells using anti-EPCAM rabbit polyclonal antibody (TA590836).