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Home Antibody All anti-EPCAM antibodies
UltraMAB

Anti-EPCAM GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590836 Rabbit Polyclonal EpCAM Antibody 100ug $325 In Stock
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WB(2)
IHC(4)
IF(1)
ELISA(1)
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Also for EPCAM (NM_002354)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein (NM_002354).
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Monkey Concentration1.1mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000, IHC 1:150, IF 1:500,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens epithelial cell adhesion molecule (EPCAM)
Alternative NameDIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1
Database LinkNP_002345
Entrez Gene 4072 Human
Entrez Gene 677680 Monkey
FunctionCD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule inthe basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has beendetected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
Related PathwayES Cell Differentiation/IPSTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPCAM.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPCAM polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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ELISA: EpCAM Antibody 19300002 - Affinity Purified
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EPCAM rabbit polyclonal antibody. (TA590836)
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Immunofluorescent staining of HeLa cells using anti-EPCAM rabbit polyclonal antibody (TA590836).
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