OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-EIF2S1 antibodies

Anti-EIF2S1 TRUEMAB Antibody clone 5A5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501374
  • anti-EIF2S1 mouse monoclonal antibody, clone 5A5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401321) , 20ug Explanation
100ul 325 In Stock
CF501374
  • Carrier-free (BSA/glycerol-free) EIF2S1 mouse monoclonal antibody, clone 5A5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
Add to Shopping Cart
spacer
WB(2)
IHC(5)
spacer
Also for EIF2S1 (NM_004094)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Clone Nameclone 5A5 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.59 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB 1:1000~2000, IHC 1:50,
Predicted MW Explanation 35.9 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1)
Alternative NameEIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Database LinkNP_004085
Entrez Gene 1965 Human
Entrez Gene 54318 Rat
Entrez Gene 710150 Monkey
Entrez Gene 480361 Dog
FunctionThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501374, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501374, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501374, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501374, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EIF2S1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501374, Dilution 1:50)

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title