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Home Antibody All anti-EIF2S1 antibodies

Anti-EIF2S1 TRUEMAB Antibody clone 3H7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501314
  • anti-EIF2S1 mouse monoclonal antibody, clone 3H7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401321) , 20ug Explanation
100ul 325 In Stock
CF501314
  • Carrier-free (BSA/glycerol-free) EIF2S1 mouse monoclonal antibody, clone 3H7
100ug $450 3-4 weeks
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WB(2)
FC(2)
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Also for EIF2S1 (NM_004094)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human EIF2S1 (NP_004085) produced in HEK293T cell.
Clone Nameclone 3H7 IsotypeIgG1
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.62 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:1000~2000, FLOW 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa (EIF2S1)
Alternative NameEIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
Database LinkNP_004085
Entrez Gene 1965 Human
Entrez Gene 54318 Rat
Entrez Gene 710150 Monkey
Entrez Gene 480361 Dog
FunctionThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF2S1 (RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF2S1 monoclonal antibody.
FC Image
HEK293T cells transfected with either RC200368 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF2S1 antibody(TA501314), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-EIF2S1 antibody(TA501314),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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