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Home Antibody All anti-EIF2AK2 antibodies

Anti-EIF2AK2 Antibody Y117

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300452
  • Rabbit Monoclonal Antibody against EIF2AK2 (Clone Y117)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400976) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for EIF2AK2 (NM_002759)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near C-terminus of human PKR was used as immunogen. The antibody does not cross-react with other GCN2 family members.
Clone NameY117 IsotypeProtein A purified
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:5000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; IP: 1:80
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), transcript variant 1
Alternative NameEIF2AK1; PKR; PPP1R83; PRKR
Database LinkNP_002750
Entrez Gene 5610 Human
FunctionProtein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2a), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3).
Related PathwayProtein KinaseTranscription FactorsDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PKR antibody [Y117]; Anti-PKR antibody [Y117] at 1/10000 dilution + MCF-7 cell lysate.Predicted band size : 62 kDa.Observed band size : 68 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - PKR antibody [Y117]; Immunohistochemical analysis of paraffin-embedded human colon carcinoma using TA300452 at 1/100 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - PKR antibody [Y117]; Immunofluorescent staining of HeLa cells using TA300452 at 1/250 dilution.

 

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