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Anti-EIF1 TRUEMAB Antibody Clone OTI2B9
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for EIF1 (NM_005801)
|Full length human recombinant protein of human EIF1 (NP_005792) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF1 (RC201271, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF1.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-EIF1 monoclonal antibody at 1:200 dilution.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-EIF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502844)
Anti-EIF1 mouse monoclonal antibody (TA502844) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EIF1(RC201271 ).
Immunofluorescent staining of HeLa cells using anti-EIF1 mouse monoclonal antibody (TA502844).
HEK293T cells transfected with either RC201271 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-EIF1 antibody(TA502844), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-EIF1 antibody(TA502844),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-EIF1 antibody(TA502844),(Red), compared to a nonspecific negative control antibody,(Blue).