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Home Antibody All anti-EGLN3 antibodies

Anti-EGLN3 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA322210
  • Anti-EGLN3 Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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IHC(1)
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Also for EGLN3 (NM_022073)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length fusion protein
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.9mg/ml
Guaranteed Application *IHC Suggested DilutionsELISA: 1:2000-5000, IHC: 1:25-100
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens egl-9 family hypoxia-inducible factor 3 (EGLN3)
Alternative NameHIFP4H3; HIFPH3; PHD3
Database LinkNP_071356
FunctionCellular oxygen sensor that catalyzes; under normoxic conditions; the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal; NODD; and C-terminal; CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions; the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus; heterodimerization with HIF1B; and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia; limiting glycolysis. Under normoxia; hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes; inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex.
Related Pathway

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IHC Image
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using EGLN3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)

 

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