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Anti-EGFR PHOSPHO Antibody
Also for EGFR (NM_005228)
|This EGFR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1172 of human EGFR.|
||WB: 1:1000; DB: 1:500
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
|This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).
Western blot analysis of extracts from A431 cells,untreated or treated with EGF using Phospho-EGFR(Tyr1172)(left) or EGFR antibody(right).
Dot blot analysis of anti-EGFR-pY1172 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.