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Home Antibody All anti-EGFR antibodies

Anti-EGFR PHOSPHO Antibody EPR2257

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA306976
  • Rabbit monoclonal antibody against EGFR Phospho (pS695) (clone EPR2257 ) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for EGFR (NM_005228)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding Serine 695 in human EGFR was used as an immunogen. The antibody detects EGFR only when phosphorylated on Serine 695.
Clone NameEPR2257 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF Suggested DilutionsICC: 1:100 - 250, WB: 1:1,000 - 5,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
FunctionEpidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that is involved in diverse cellular functions including cell proliferation, differentiation, motility, survival and in tissue development (1, 2). EGFR is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/ phospholipid-dependent enzyme) mediated phosphorylation at identified sites. EGFR contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which account for the majority of phosphate covalently bound to the receptor in vivo. Analysis of predicted secondary structure of EGFR reveals that all of the phosphorylation sites are located near beta turns (3).
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EGFR (phospho S695) antibody [EPR2257]; All lanes : Anti-EGFR (phospho S695) antibody [EPR2257] at 1/1000 dilution.Lane 1 : A431 cell lysate untreated.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - EGFR (phospho S695) antibody [EPR2257]; Immunofluorescent staining of A431 cells using TA306976 at 1/100 dilution.

 

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