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Home Antibody All anti-EGFR antibodies

Anti-EGFR PHOSPHO Antibody E124

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA306974
  • Rabbit monoclonal antibody against EGFR Phospho (pY1173)(clone E124) (Phospho-Specific)
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(2)
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Also for EGFR (NM_005228)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of human EGF receptor was used as immunogen. The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor form
Clone NameE124 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC: 1:25-50, IHC: 1:25-50, IP: 1:10, WB: 1:1,000-10,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
FunctionEpidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). Once activated, EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Addtionally, phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR (4).
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

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WB Image
Western blot - Anti-EGFR (phospho Y1173) antibody [E124]; All lanes : Anti-EGFR (phospho Y1173) antibody [E124] at 1/1000 dilution.Lane 1 : A431.Lane 2 : A431 + EGF cell lysates.Predicted band size : 170 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1173) antibody [E124]; TA306974 (at 1/50) staining (A) untreated and (B) Phosphatase-treated skin carcinoma tissue by IHC-P.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124]; Immunofluorescent staining of untreated A431 cells using TA306974.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124]; Immunofluorescent staining of Phosphatase-treated A431 cells using TA306974.

 

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