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Home Antibody All anti-EGFR antibodies

Anti-EGFR PHOSPHO Antibody EP2256Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303493
  • Rabbit monoclonal antibody against EGFR Phospho (pT693) (EP2256Y ) (phospho-specific)
100ul $325 3-7 Days
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WB(1)
IF(1)
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Also for EGFR (NM_005228)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding threonine 693 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on thr693.
Clone NameEP2256Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IF Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:10000 - 1:20000; IP: 1:40
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
FunctionThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EGFR (phospho T693) antibody [EP2256Y]; All lanes : Anti-EGFR (phospho T693) antibody [EP2256Y] at 1/20000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 175 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - EGFR (phospho T693) antibody [EP2256Y]; TA303493 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.

 

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