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Anti-EGFR PHOSPHO Antibody EP2256Y
Also for EGFR (NM_005228)
|A phospho specific peptide corresponding to residues surrounding threonine 693 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on thr693.|
||Tissue culture supernatant
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||ICC/IF: 1:100 - 1:250; WB: 1:10000 - 1:20000; IP: 1:40
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt or IHC-P.
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Western blot - EGFR (phospho T693) antibody [EP2256Y]; All lanes : Anti-EGFR (phospho T693) antibody [EP2256Y] at 1/20000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with EGF.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 175 kDa .
Immunocytochemistry/ Immunofluorescence - EGFR (phospho T693) antibody [EP2256Y]; TA303493 at 1/100 dilution staining EGFR in A431 cells by Immunofluorescence.