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Anti-EGFR PHOSPHO Antibody EP2258IHCY
Also for EGFR (NM_005228)
|A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 1110 of human EGF receptor was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1,000 - 5,000; ICC: 1:100 - 250; IP: 1:40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq]. |
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - EGFR (phospho Y1110) antibody [EP2258IHCY]; All lanes : Anti-EGFR (phospho Y1110) antibody [EP2258IHCY] at 1/5000 dilution.Lane 1 : A431 cell lysate, untreated.Lane 2 : A431 cell lysate, treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugatecd Goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.Observed band size : 200 kDa .
Immunocytochemistry/ Immunofluorescence - EGFR (phospho Y1110) antibody [EP2258IHCY]; A431 cells stained with ab68470 at 1/100 dilution.