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Home Antibody All anti-EGF antibodies

Anti-EGF GAT Antibody Polyclonal

Generated by Genomic Immunization


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA590718 Rabbit Polyclonal EGF Antibody 100ug $325 In Stock
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OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.2mg/ml
Guaranteed Application *WB, ELISA, DB Suggested Dilutions WB 1:20000,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens epidermal growth factor (EGF), transcript variant 1
Alternative NameHOMG4; URG
Database LinkNP_001954
Entrez Gene 1950 Human
FunctionEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Related PathwayES Cell Differentiation/IPSTransmembraneAdult stem cellsEmbryonic stem cellsInduced pluripotent stem cellsDruggable Genome MAPK signaling pathwayErbB signaling pathwayCytokine-cytokine receptor interactionEndocytosisFocal adhesionGap junctionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGF (RC210817, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGF.
ELISA: EGF Antibody
Dot Blot: EGF Antibody - The anti-EGF antibody (left quadruplicate spots at each panel) or control antibody (right quadruplicate spots at each panel) were printed on membranes at 10 ng/spot. The membranes were incubated with biotinylated cell lysate (50 ug per membrane), followed by probing with streptavidin-HRP and CCD camera image acquisition. (a), Cell lysate from Jurkat cells; (b), Cell lysate from 293T cells. Data courtesy of a SDI customer.
Dot Blot: EGF Antibody - Protein samples (left spot: 293T cell lysate; right spot: Jurkat cell lysate) were printed on membranes at 40 ng/spot. The membrane was incubated with anti-EGF antibody (1:4000 dilution) and then probed anti-rabbit IgG-HRP. Signals were acquired with a CCD camera. Data courtesy of a SDI customer.


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