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Anti-EEF2K Antibody EP724Y
Also for EEF2K (NM_013302)
|A synthetic peptide corresponding to residues in the Calmodulin binding region of human eEF2 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:50000; IP: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens eukaryotic elongation factor-2 kinase (EEF2K)|
|Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively by phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6). |
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Western blot - EEF2K antibody [EP724Y]; All lanes : Anti-EEF2K antibody [EP724Y] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 82 kDa.Observed band size : 95 kDa .