Anti-DOK7 Antibody, Clone 1A9

Also for DOK7 (NM_173660)

OriGene Data

Immunogen	Full length human recombinant protein of human DOK7(NP_775931) produced in HEK293T cell.
Clone Name	Clone 1A9	Isotype	IgG1
Species Reactivity	Human , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens docking protein 7 (DOK7), transcript variant 1
Alternative Name	C4orf25; CMS1B
Database Link	NP_775931
Function	The protein encoded by this gene is essential for neuromuscular synaptogenesis. The protein functions in aneural activation of muscle-specific receptor kinase, which is required for postsynaptic differentiation, and in the subsequent clustering of the acetylcholine receptor in myotubes. This protein can also induce autophosphorylation of muscle-specific receptor kinase. Mutations in this gene are a cause of familial limb-girdle myasthenia autosomal recessive, which is also known as congenital myasthenic syndrome type 1B. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOK7 (RC219267, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOK7.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-DOK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-DOK7 mouse monoclonal antibody. (TA504801)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOK7 mouse monoclonal antibody. (TA504801)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-DOK7 mouse monoclonal antibody. (TA504801)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DOK7 mouse monoclonal antibody. (TA504801)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-DOK7 mouse monoclonal antibody. (TA504801)

IF Image

Anti-DOK7 mouse monoclonal antibody (TA504801) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DOK7(RC219267).

FC Image

HEK293T cells transfected with either RC219267 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DOK7 antibody(TA504801), and then analyzed by flow cytometry.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
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DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-DOK7 TrueMAB Antibody Clone 1A9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data