OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody UltraMAB

UltraMAB

DOCK2 UltraMAB Antibody, Clone UMAB142

UltraMAB™ Antibodies - Validated with 10K Protein Chip

div

 
Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800039
  • DOCK2 mouse monoclonal antibody,clone UMAB142, 100 ul
$468 In Stock Add to Shopping Cart
UM870039
  • DOCK2 mouse monoclonal antibody,clone UMAB142, 30 ul
$120 In Stock Add to Shopping Cart
WB(2)
Data Img
IHC(4)
Data Img
IF(1)
Data Img
Write Review & Get Rewarded
X

Write a review and get rewarded!

Review this antibody and earn an OriGene coupon or Amazon giftcard

Follow these easy steps to reward yourself:

  1. Sign in to your account, or register for a new account;
  2. Write a review;
  3. Receive a coupon or giftcard once your review is accepted.

Click the button to start writing a review


OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1544-1830 of human DOCK2 (NP_004937) produced in E.coli.
Clone NameClone UMAB142 IsotypeIgG2a
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:2000, IHC 1:150,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens dedicator of cytokinesis 2 (DOCK2)
Alternative NameFLJ46592; KIAA0209
Database LinkNP_004937
FunctionThe protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells, predominantly in the peripheral blood leukocytes, and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration, through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts, suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq, Oct 2011].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of  Jurkat cell lysate (35ug) by using anti-DOCK2 monoclonal antibody.
spacer
Western blot analysis of RPMI8226 cell lysate (35ug) by using anti-DOCK2 monoclonal antibody.
spacer
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DOCK2 mouse monoclonal antibody. (UM800039; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
spacer
Immunohistochemical staining of paraffin-embedded human lung cancer using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.
spacer
Immunohistochemical staining of paraffin-embedded human melanoma using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.
spacer
Immunohistochemical staining of paraffin-embedded human tonsil using anti-DOCK2 clone UMAB142 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. UM800039 requires HIER with with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. IHC staining shows germinal and nongerminal center with strong stain in the cytoplasm and membrane.
spacer
Immunofluorescent staining of Jurkat cells using anti-DOCK2 mouse monoclonal antibody (UM800039, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title