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Also for DLL4 (NM_019074)
|Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.|
|human, partial reactivity to mouse and rat
||Lot dependent; please refer to CoA along with shipment
||ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,500, IHC: 10 ug/mL to 20 ug/mL
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.
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Western Blot of Rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 µg per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.
Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.