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Anti-DFFA TRUEMAB Antibody clone 3F12
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for DFFA (NM_004401)
|Full length human recombinant protein of human DFFA (NP_004392) produced in E.coli.|
||WB 1:200~500, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1|
|DFF-45; DFF1; ICAD|
|Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody.
Anti-DFFA mouse monoclonal antibody (TA500063) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA(RC202879).