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Home Antibody All anti-DFFA antibodies

Anti-DFFA TRUEMAB Antibody Clone OTI1B10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500061 anti-DFF45 (ICAD) mouse monoclonal antibody, clone OTI1B10 (formerly 1B10) 100ul $325 In Stock
LC418008 DFFA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500061 Carrier-free (BSA/glycerol-free) DFF45 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(3)
IF(2)

OriGene Data

ImmunogenRecombinant protein expressed in E.coli corresponding to amino acids 1-331 of human DFF45
Clone NameClone OTI1B10 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500, IF (1:100)
Predicted MW Explanation 36.3 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1
Alternative NameDFF-45; DFF1; ICAD
Database LinkNP_004392
Entrez Gene 1676 Human
FunctionApoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene
Related Pathway Apoptosis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA(RC202879) (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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DFF45 antibody (1B10) at 1:500 dilution + Hela cell lysate
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Anti-DFFA mouse monoclonal antibody (TA500061) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY DFFA(RC202879)
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Immunofluorescent staining of HeLa cells using anti-DFFA mouse monoclonal antibody (TA500061).
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