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Home Antibody All anti-DFFA antibodies

Anti-DFFA TRUEMAB Antibody clone 1B10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA500061
  • anti-DFF45 (ICAD) mouse monoclonal antibody, clone 1B10
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418008) , 20ug
100ul $325 In Stock
CF500061
  • Carrier-free (BSA/glycerol-free) DFF45 mouse monoclonal antibody,clone 1B10
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IF(2)
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Also for DFFA (NM_004401)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenRecombinant protein expressed in E.coli corresponding to amino acids 1-331 of human DFF45
Clone Nameclone 1B10 IsotypeIgG1
Species ReactivityHuman Concentration0.42mg/ml
Guaranteed Application *WB, IF Suggested DilutionsWB: 1:500, IF (1:100)
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1
Alternative NameDFF-45; DFF1; ICAD
Database LinkNP_004392
FunctionApoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene
Related Pathway
Apoptosis

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA(RC202879) (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA.
WB Image
DFF45 antibody (1B10) at 1:500 dilution + Hela cell lysate
IF Image
Anti-DFFA mouse monoclonal antibody (TA500061) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY DFFA(RC202879)
IF Image
Immunofluorescent staining of HeLa cells using anti-DFFA mouse monoclonal antibody (TA500061).

 

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