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Also for DFFA (NM_004401)
|DFF45 antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45.|
||ELISA, WB: 0.5 - 1 ug/ml, ICC: 5 ug/ml
|PBS containing 0.02% sodium azide.|
|Antibody is DEAE purified
|Homo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1|
|DFF-45; DFF1; ICAD|
|Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.|
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Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.
Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 ug/mL.