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Home Antibody All anti-DDX5 antibodies

Anti-DDX5 Antibody EPR7239

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA310402 Rabbit Monoclonal antibody against DDX5 100ul $325 In Stock
LC401398 DDX5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(2)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human DDX5 was used as an immunogen.
Clone NameEPR7239 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 69 kDa
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5)
Alternative NameG17P1; HLR1; HUMP68; p68
Database LinkNP_004387
Entrez Gene 1655 Human
Entrez Gene 13207 Mouse
Entrez Gene 287765 Rat
FunctionDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX5 is a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen (1).
Related Pathway Spliceosome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Anti-DDX5 antibody [EPR7239]; All lanes : Anti-DDX5 antibody [EPR7239] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : A431 cell lysate.Lane 4 : HT29 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 69 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7239]; TA310402, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX5 antibody [EPR7239]; TA310402, at a dilution of 1/250, staining DDX5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
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Immunocytochemistry/ Immunofluorescence - Anti-DDX5 antibody [EPR7239]; TA310402, at a dilution of 1/100, staining DDX5 in HeLa cells by Immunofluorescence.
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