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Also for DDX5 (NM_004396)
|Peptide with sequence C-PMIGYPMPTGYSQ, from the C Terminus of the protein sequence according to NP_004387.1.|
|Test: Human. Expected from seq similarity: Human, Mouse, Rat, Dog
||Lot dependent; please refer to CoA along with shipment
||WB: 0.3-1ug/mL; IHC: 2-5ug/mL
|Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.|
|Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
|Homo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 5 (DDX5)|
|G17P1; HLR1; HUMP68; p68|
Entrez Gene 1655 Human
Entrez Gene 13207 Mouse
Entrez Gene 287765 Rat
Entrez Gene 480472 Dog
|DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq].|
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TA302658 (0.3µg/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302658 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.