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Home Antibody All anti-DDX3X antibodies

Anti-DDX3X Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA302080 Rabbit Polyclonal Antibody against DDX3X (C-term) 100ug $325 3-7 Days
LC419970 DDX3X HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
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Also for DDX3X (NM_001356)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis DDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-485 amino acids from the C-terminal region of human DDX3.
Clone Name IsotypeRabbit Ig
Species ReactivityHuman (Predicted: Mouse, Xenopus) Concentration0.25 mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000, IHC: 1:10~50
Predicted MW Explanation 73113 Da
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked (DDX3X), transcript variant 1
Alternative NameCAP-Rf; DBX; DDX14; DDX3; HLP2
Database LinkNP_001347
Entrez Gene 1654 Human
Entrez Gene 13205 Mouse
FunctionDEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 is a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. The DDX3 gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog.
Related PathwayES Cell Differentiation/IPS RIG-I-like receptor signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody (C-term) (Cat.#TA302080).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).
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Western blot analysis of DDX3 Antibody (C-term) (Cat.#TA302080) in Hela cell line lysates (35ug/lane). DDX3 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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