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Home Antibody All anti-DAP3 antibodies

Anti-DAP3 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA302460
  • Goat Polyclonal Antibody against DAP3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403256) , 20ug
100ug 325 3-7 Days
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WB(3)
IF(1)
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Also for DAP3 (NM_033657)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1; NP_004623.1.
Clone Name IsotypeGoat IgG
Species ReactivityHuman Concentration0.5 mg/ml
Guaranteed Application *WB, IF Suggested DilutionsELISA: 1:16,000; WB: 1-3 ug/ml; ICC: 3-10ug/ml
BufferSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Reference Data

Target NameHomo sapiens death associated protein 3 (DAP3), transcript variant 1
Alternative NamebMRP-10; DAP-3; MRP-S29; MRPS29
Database LinkNP_387506
FunctionMammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq].
Related Pathway

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WB Image
TA302460 staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
WB Image
HEK293 overexpressing DAP3 (Cat# RC223182) and probed with TA302460 (mock transfection in first lane), tested by Origene.
WB Image
TA302460 (1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
IF Image
TA302460 (0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.

 

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