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Home Antibody All anti-DACH1 antibodies

Anti-DACH1 Antibody EPR2841Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307015
  • Rabbit monoclonal antibody against Dachshund1 (clone EPR1427Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC409071) , 20ug Explanation
100ul 325 In Stock
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WB(1)
FC(1)

OriGene Data

Immunogen A synthetic peptide corresponding to residues near the C-terminus of human Dachshund1 was used as an immunogen.
Clone NameEPR2841Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsFC: 1:120, IP: 1:40, WB: 1:500 - 1000,
Predicted MW Explanation 79 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens dachshund family transcription factor 1 (DACH1), transcript variant 1
Alternative NameDACH
Database LinkNP_542937
Entrez Gene 1602 Human
Entrez Gene 13134 Mouse
FunctionDachshund is a human homologue of Drosophila melanogaster dachshund, which is a nuclear factor essential for determining cell fates in the eye, leg, and nervous system. Mouse Dachshund protein was immunolocalized to specific cell types within the developing kidneys, eyes, cochleae, and limb buds (1). Dachshund was found to be expressed in breast cancer cell lines and to inhibit transforming growth factor-beta (TGF-beta)-induced apoptosis. Dachshund participates in the negative regulation of TGF-beta signaling by interacting with NCoR and Smad4 (2).
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - DACH1 antibody [EPR2841Y]; All lanes : Anti-DACH1 antibody [EPR2841Y] at 1/1000 dilution.Lane 1 : MCF-7 cell lysate.Lane 2 : Hela cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 79 kDa.Observed band size : 97 kDa .
FC Image
Flow Cytometry-Anti-DACH1 antibody [EPR2841Y](TA307015); Overlay histogram showing HeLa cells stained with TA307015 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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