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Anti-DACH1 Antibody EPR2841Y
Also for DACH1 (NM_080759)
| A synthetic peptide corresponding to residues near the C-terminus of human Dachshund1 was used as an immunogen.|
|Mouse, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
||FC: 1:120, IP: 1:40, WB: 1:500 - 1000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens dachshund family transcription factor 1 (DACH1), transcript variant 1|
Entrez Gene 1602 Human
Entrez Gene 13134 Mouse
|Dachshund is a human homologue of Drosophila melanogaster dachshund, which is a nuclear factor essential for determining cell fates in the eye, leg, and nervous system. Mouse Dachshund protein was immunolocalized to specific cell types within the developing kidneys, eyes, cochleae, and limb buds (1). Dachshund was found to be expressed in breast cancer cell lines and to inhibit transforming growth factor-beta (TGF-beta)-induced apoptosis. Dachshund participates in the negative regulation of TGF-beta signaling by interacting with NCoR and Smad4 (2). |
|Transcription Factors |
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Western blot - DACH1 antibody [EPR2841Y]; All lanes : Anti-DACH1 antibody [EPR2841Y] at 1/1000 dilution.Lane 1 : MCF-7 cell lysate.Lane 2 : Hela cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 79 kDa.Observed band size : 97 kDa .
Flow Cytometry-Anti-DACH1 antibody(TA307015); Overlay histogram showing HeLa cells stained with TA307015 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). This antibody gave a positive signal in HeLa cells under the same conditions.