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Anti-CUL4B Antibody EPR3200
Also for CUL4B (NM_003588)
|A synthetic peptide corresponding to residues in human CUL4A/B was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2,000~10,000; IHC: 1: 100~250; FC: 1:60
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens cullin 4B (CUL4B), transcript variant 1|
|CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2|
Entrez Gene 8450 Human
Entrez Gene 72584 Mouse
Entrez Gene 302502 Rat
|Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes, including regulating replication and transcription (1-2). Two cullin 4 (CUL4) proteins, CUL4A and CUL4B, have been identified. CUL4A and CUL4B are two closely related proteins, coded by two different genes. The amino acid sequences of CUL4A and CUL4B show 80% similarity, differing by the unique and extended N-terminus of CUL4B. CUL4B is reported to be found in the nucleus as well as the cytoplasm during differentiation, while CUL4A has been reported to be present predominantly in the cytoplasm. CUL4B is suggested to have a more specific role than CUL4A due to the presence of functional nuclear localization signal sequence in the N-terminus. CUL4A is noted as essential for embryonic development (3). |
| Nucleotide excision repairUbiquitin mediated proteolysis|
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Western blot - Cullin 4A/B antibody [EPR3200]; All lanes : Anti-Cullin 4A/B antibody [EPR3200] at 1/5000 dilution.Lane 1 : Fetal uterus lysate.Lane 2 : Hela lysate.Lane 3 : A375 lysate.Lane 4 : Fetal testis lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 88,102 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cullin 4A/B antibody [EPR3200]; TA301340 at 1/100 dilution staining Cullin 4B in human brain tissue, using a HRP/AP polymerized secondary antibody.