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Anti-CTCF Antibody


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SKU Description Amount Price Availability*  
  • Rabbit polyclonal anti-CTCF antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416554) , 20ug Explanation
100ug $325 3-7 Days
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OriGene Data

ImmunogenCTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000-1:50,000, WB: 1:500-1:2:000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Transcriptional repressor CTCF (also known as CCCTC-binding factor) is a transcription factor encoded by the CTCF gene. The CTCF, and the closely related Brother of the Regulator of Imprinted Sites (BORIS), are highly conserved zinc finger proteins implicated in diverse regulatory functions, including transcriptional activation/repression, insulation, imprinting, and X chromosome inactivation. Expression of BORIS is restricted to spermatocytes and is mutually exclusive of CTCF expression. CTCF is ubiquitously expressed in higher eukaryotes and contains a highly conserved and eleven zinc finger central DNA-binding domain, having very high homology between mouse, chicken, and human and is embedded within slightly divergent N and C termini. CTFC plays a critical role in the epigenetic regulation and chromatin remodeling. CTCF has been reported to bind to a variety of DNA target sites that perform distinct functions, including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting.

Reference Data

Target NameHomo sapiens CCCTC-binding factor (zinc finger protein) (CTCF), transcript variant 1
Alternative NameMRD21
Database LinkNP_006556
Entrez Gene 10664 Human
Entrez Gene 13018 Mouse
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of Rabbit Anti-CTCF antibody. Lane 1: H929 nuclear cell lysates. Load: 30 µg per lane. Primary antibody: CTCF antibody at 1.2 µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 3% BSA overnight at 4°C. Predicted/Observed size: 82.8 kDa or 150 kDa for CTCF. Other band(s): CTCF splice variants.


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