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Home Antibody All anti-Chk2 antibodies

Anti-Chk2 Antibody EPR5528

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310327
  • Rabbit Monoclonal antibody against Chk2
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC423638) , 20ug
100ul 325 3-7 Days
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WB(1)
Assay(1)
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Also for Chk2 (NM_001005735)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues at the N-terminus of human Chk2 was used as an immunogen.
Clone NameEPR5528 IsotypeTissue culture supernatant
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ASSAY Suggested DilutionsWB: Use at an assay dependent dilution
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Reference Data

Target NameHomo sapiens checkpoint kinase 2 (CHEK2), transcript variant 3
Alternative NameCDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Database LinkNP_001005735
FunctionChk2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, Chk2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in Chk2 have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this protein are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors (1).
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Chk2 antibody [EPR5528]; All lanes : Anti-Chk2 antibody [EPR5528] at 1/10000 dilution.Lane 1 : SH-SY5Y lysate.Lane 2 : HeLa lysate.Lane 3 : HT29 lysate.Lane 4 : 293T cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat-anti-rabbit HRP at 1/2000 dilution.Predicted band size : 61 kDa.Observed band size : 61 kDa.
Assay Image
Other-Anti-Chk2 antibody [EPR5528](TA310327); Equilibrium disassociation constant (KD)..

 

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