Anti-CFLAR Antibody,

Also for CFLAR (NM_001127183)

OriGene Data

Immunogen	FLIP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human FLIPaFLIPl form.
Clone Name		Isotype	IgG
Species Reactivity	Human, Mouse, Rat	Concentration	1ug/ul
Guaranteed Application *	WB, IF	Suggested Dilutions	ELISA, WB: 1 ug/ml, ICC: 10 ug/ml, IF: 10 ug/ml
Buffer	PBS containing 0.02% sodium azide.
Purification	Antibody is DEAE purified (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens CASP8 and FADD-like apoptosis regulator (CFLAR), transcript variant 2
Alternative Name	c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CLARP; FLAME; FLAME-1; FLAME1; FLIP;
Database Link	NP_001120655
Function	Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIPalpha, beta, gamma, and delta, respectively (8).
Related Pathway	Apoptosis

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WB Image

Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution.

IF Image

Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 ug/mL.

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
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PE
FITC
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DyLight® 633

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-CFLAR Antibody

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data